[Histonet] Thin sectioning bone in PMMA

mwstarbu <@t> mdanderson.org mwstarbu <@t> mdanderson.org
Wed Aug 1 13:10:40 CDT 2007

We apply a 50% butyl glycol, 35% ethanol solution to the sections.   The 
application is as follows.  We apply a liberal amount with a small paint 
brush and let dry 5 -10 minutes under a fume hood.  Apply again and use 
downward pressure with the brush to remove wrinkles. During the 2nd 
application you will notice the sections are much more pliable enableing 
you to easily remove large wrinkles.  We then cover the slides with 
plastic strips, press and dry at 55 degrees C overnnight


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