[Histonet] Thin sectioning bone in PMMA
mwstarbu <@t> mdanderson.org
mwstarbu <@t> mdanderson.org
Wed Aug 1 13:10:40 CDT 2007
We apply a 50% butyl glycol, 35% ethanol solution to the sections. The
application is as follows. We apply a liberal amount with a small paint
brush and let dry 5 -10 minutes under a fume hood. Apply again and use
downward pressure with the brush to remove wrinkles. During the 2nd
application you will notice the sections are much more pliable enableing
you to easily remove large wrinkles. We then cover the slides with
plastic strips, press and dry at 55 degrees C overnnight
Mike
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