AW: [Histonet] CD4 (4B12) protocol on Benchmark XT

[Gudrun Lang]

What are the specifications of your protocol?

CC1 mild or standard

Antibody-dilution?

 

I use the ultra-view polymer kit; CD4 (4B12) from Novocastra.

Best regards

 

Gudrun Lang

 

Biomed. Analytikerin

Histolabor

Akh Linz

Krankenhausstr. 9

4020 Linz

+43(0)732/7806-6754

  _____  

Von: Vivian Anderson [mailto:vivanderson14 <@t> hotmail.com] 
Gesendet: Montag, 30. April 2007 19:53
An: gu.lang <@t> gmx.at
Betreff: RE: [Histonet] CD4 (4B12) protocol on Benchmark XT

 

Hi, 

I have a protocol for C4d on Ventana Benchmark. It works great!

I deparaffinize on line and use CC1 Standard.

Incubate for 26 minutes.

Amplify

Use A/B Block

and counterstain.

Good luck!

Vivian Anderson, HT(ASCP)
Lead Histotech
Memorial Medical Center

Springfield, Illinois



 

 


  _____  


From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Reply-To: gu.lang <@t> gmx.at
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] CD4 (4B12) protocol on Benchmark XT
Date: Mon, 30 Apr 2007 18:08:12 +0200
Hi,

can someone provide me with a successfull protocol for CD4 clone 4B12 for
the Benchmark XT?



Thanks in advance

Gudrun

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