AW: [Histonet] CD4 (4B12) protocol on Benchmark XT

Gudrun Lang gu.lang <@t>
Mon Apr 30 14:55:37 CDT 2007

What are the specifications of your protocol?

CC1 mild or standard



I use the ultra-view polymer kit; CD4 (4B12) from Novocastra.

Best regards


Gudrun Lang


Biomed. Analytikerin


Akh Linz

Krankenhausstr. 9

4020 Linz



Von: Vivian Anderson [mailto:vivanderson14 <@t>] 
Gesendet: Montag, 30. April 2007 19:53
An: gu.lang <@t>
Betreff: RE: [Histonet] CD4 (4B12) protocol on Benchmark XT



I have a protocol for C4d on Ventana Benchmark. It works great!

I deparaffinize on line and use CC1 Standard.

Incubate for 26 minutes.


Use A/B Block

and counterstain.

Good luck!

Vivian Anderson, HT(ASCP)
Lead Histotech
Memorial Medical Center

Springfield, Illinois




From: "Gudrun Lang" <gu.lang <@t>>
Reply-To: gu.lang <@t>
To: <histonet <@t>>
Subject: [Histonet] CD4 (4B12) protocol on Benchmark XT
Date: Mon, 30 Apr 2007 18:08:12 +0200

can someone provide me with a successfull protocol for CD4 clone 4B12 for
the Benchmark XT?

Thanks in advance


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