[Histonet] Proper Decalcification

Fri Apr 27 10:38:44 CDT 2007

How to use the FAXITRON for decalcification endpoint checks is found in 
Gamble and Bancroft Theory and Practice of Histological Technique, 5th 
edition, 2001, Bone Chapter.  The instrument is not cheap but it is the 
most sensitive way to do a decalcification endpoint.  Finding film cans 
sometimes be a chore, but you can xray many samples at once to make it more 
efficient.

  There are other ways to do decalcification endpoints, some simple but 
very effective.  The chemical test is easy, cheap and commonly done, found 
in histotechnology textbooks.  Another is a weight loss/weight gain 
originally done to check nitric acid decalcification.  Since we gave away 
our FAXITRON (worst thing ever done!) we do the weight loss/weight gain 
method, but you need a balance that weighs in milligrams.   I will be happy 
to send the latter two privately if needed.

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610

At 05:21 AM 4/27/2007, you wrote:
>I looked up info. on it at www.faxitron.com
>
>
>Jeanine Bartlett
>Infectious Disease Pathology Branch
>(404) 639-3590
>jeanine.bartlett <@t> cdc.hhs.gov
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu 
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of LNLJ (Lene 
>Lyngsie Jensen)
>Sent: Friday, April 27, 2007 7:01 AM
>To: Molinari, Betsy; histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] Proper Decalcification
>
>Hey all...
>
>I have never heard about this machine before. I don't think we have it 
>here I Denmark (small country in Scandinavia:-)).
>
>Could someone please tell me a bit more about it, and how you use it? 
>Maybe it would be worth getting it all the way to Denmark?
>
>
>
>Thanks.
>
>
>Lene Lyngsie Jensen
>Medical Laboratory Scientific Officer (MLSO**)
>GLP-1 and Obesity Pharmacology
>
>Novo Nordisk A/S
>Novo Nordisk Park
>DK-2760 Måløv
>Denmark
>
>
>
>
>
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu 
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy
>Sent: 16. april 2007 15:32
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] Proper Decalcification
>
>
>
>Faxitron MX 20
>
>
>
>Betsy Molinari HT (ASCP)
>
>Texas Heart Institute
>
>Cardiovascular Pathology
>
>6770 Bertner Ave.
>
>Houston,TX 77030
>
>832-355-6524
>
>832-355-6812 (fax)
>
>
>
>
>
>-----Original Message-----
>
>From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7 <@t> cdc.gov]
>
>Sent: Monday, April 16, 2007 5:47 AM
>
>To: Molinari, Betsy
>
>Subject: RE: [Histonet] Proper Decalcification
>
>
>
>Which model do you use?
>
>
>
>Thanks,
>
>
>
>Jeanine Bartlett, BS, HT(ASCP)QIHC
>
>Centers for Disease Control and Prevention
>
>Infectious Diseases Pathology Branch
>
>1600 Clifton Road, MS/G-32
>
>18/SB-114
>
>Atlanta, GA  30333
>
>(404) 639-3590
>
>jeanine.bartlett <@t> cdc.hhs.gov
>
>
>
>
>
>-----Original Message-----
>
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>
>Molinari, Betsy
>
>Sent: Monday, April 16, 2007 6:41 AM
>
>To: histonet <@t> lists.utsouthwestern.edu
>
>Subject: RE: [Histonet] Proper Decalcification
>
>
>
>We use the Faxitron.
>
>
>
>Betsy Molinari HT (ASCP)
>
>Texas Heart Institute
>
>Cardiovascular Pathology
>
>6770 Bertner Ave.
>
>Houston,TX 77030
>
>832-355-6524
>
>832-355-6812 (fax)
>
>
>
>
>
>-----Original Message-----
>
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
>
>Lucas
>
>Sent: Friday, April 13, 2007 10:41 AM
>
>To: histonet <@t> pathology.swmed.edu
>
>Subject: [Histonet] Proper Decalcification
>
>
>
>I've been having problems with my PA when it comes to proper
>
>decalcification of our bone specimens.  They are either over or under
>
>decalcified.
>
>
>
>Do many of you use a chemical end point method to determine the
>
>decalcification or do you use other methods?  If so, could you tell me
>
>the best product to use?
>
>
>
>I appreciate any feedback.
>
>
>
>Paula Lucas
>
>Lab Manger
>
>Bio-Path Medical Group
>
>
>
>
>
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