[Histonet] Shredding and Vacuoles in frozen sections of skin

Mickie Johnson mickie25 <@t> netzero.net
Thu Apr 26 11:07:38 CDT 2007

Hi Eva,

I train Mohs techs and have seen many artifacts. I am familiar with the
Leica 1510S as well. I feel that this is probably the way you are freezing
the specimen. I can tell more if I can learn the complete process you use.
(how you embed, etc).  It would be easier over the phone. Give me a call
whenever it is convenient.

Best regards,

Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
Web: www.mohshistotemp.com & www.mohslabstaffing.com 
Email: mickie25 <@t> netzero.net

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eva Brosco
Sent: Wednesday, April 25, 2007 10:20 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Shredding and Vacuoles in frozen sections of skin

Hello Everyone,
I am the histologist in a new Mohs surgery practice and am seeing
terrible artifact in the frozen tissue. It is multi complex (maybe
simple) in that the dermis is often blasted apart, glands are vacuoled
and not staining and the cells look spindled. I believe most is a result
of moisture (my lab is 10 feet from the autoclave room). I am using the
AccuEdge high profile blades in a Leica CM150S at - 20. I use Tissue Tek
OCT, tissue to matrix, not flat embedding technique (will try soon, its
is a good technique) Plus slides at room temp. I have scoped the slide
prestain and the artifact is present so it is not stain reagents. The
DermPath surgeon is not cauterizing until afte tissue is totally removed
from patient. I believe ice crystalls are the culprit but would like to
hear opinions. I have looked at tissue artifact on line but have not
seen any this degraded. Thank you all in advance.

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