[Histonet] c-fos staining

[Stacey Barrick]

Hi Jaina, 
   
  I did c-fos for a number of years in my old position. Just fix and then put the tissue into 30% sucrose overnight at 4degC before you freeze it for sectioning. We never had a problem and would keep the tissue forever. 

jaina.negandhi <@t> sickkids.ca wrote:
  I will be carrying out c-fos straining, and according to the protocol I am
following, I need to preserve my tissue in a mixture of succrose and
Thermosol. Thermosol is a preservative, however, I can't seem to find it
anywhere (i.e sigma, fluka etc.) Is there another chemical/preservative
that I can use instead, or even another technique?

Jaina


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


       
---------------------------------
Ahhh...imagining that irresistible "new car" smell?
 Check outnew cars at Yahoo! Autos.