[Histonet] how to keep toenail on the slide

Joe Nocito jnocito <@t> satx.rr.com
Tue Apr 24 18:37:42 CDT 2007

Joe the Toe here. I believe I can ass ist you here. (oops, I know I just mad 
someone mad, but, it wouldn't be me if I didn't).
Have you tried positive charged slides? Do you soak your nails in a solution 
before cutting?
We soak our nails in 20% sodium hydroxide (thank you Rene), then cut them 
and place on Plus Slides. We place the slides upright in an 80 C oven for 15 
minutes and let the slides cool completely before staining. Need to make 
sure that all the water is out. Hope this helps.


----- Original Message ----- 
From: "curt tague" <opiecurt <@t> yahoo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, April 24, 2007 11:47 AM
Subject: [Histonet] how to keep toenail on the slide

>i keep having my sections float off, not all but most. any suggestions?
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