[Histonet] AFB Contamination

Bryan Hewlett bhewlett <@t> cogeco.ca
Tue Apr 24 15:19:39 CDT 2007


Contaminated waterbaths, tap water rinses and re-used staining solutions 
have all been found to be a potential source of contamination.
I would suspect tap nozzles as the #1 source.


----- Original Message ----- 
From: <Tracey.Lenek <@t> CLS.ab.ca>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, April 24, 2007 4:13 PM
Subject: [Histonet] AFB Contamination


We are experiencing transient false positive ZN staining on skin biopies. 
Today a slide had clumped AFB over the majority of the slide where no tissue 
was present.  Previous to this, only one or two bacilli were noted on the 
Has anyone investigated and found a contaminated waterbath or perhaps the 
Kinyoun's is the culprit as our  lab reuses both?  Any help would be 


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