[Histonet] Re: Aqueous mountant suggestions please
anh2006 <@t> med.cornell.edu
Tue Apr 24 13:08:05 CDT 2007
We don't heat Faramount and it works well for our chromagens such as
AEC+. And like Gayle's lab, for DAB+ frozens we dehydrate and clear
and get beautiful, permanent results. Highly recommended.
At 10:34 AM -0600 4/24/07, Gayle Callis wrote:
>The Faramount works, but it must be heated before coverglass
>application, and the heating adds another step you have to deal
>with, Howevere, it is very nice aqueous media that sets up hard and
>We have also done frozen sections with DAB and set up the
>dehydration/clearing for permanent mounting media. I must admit
>this gives a superb final preparation that when dry, the mounting
>media doesn't retract under the coverglass. It is not that
>difficult to set up the dehyration/clearing/permanent mountant, and
>there are some nice mini-staining devices to help you out depending
>on how many slides you stain at one time. We prefer
>dehydration/clearing/and permanent media with DAB.
>At 09:39 AM 4/24/2007, you wrote:
>>I need and aqueous mountant for immunohistochemistry slides. I am
>>currently using Vectamount AQ from Vectashield but am having some
>>problems with it not hardening completely and it doesnt seem to last
>>very long ie. the use by date is already up and I'm only halfway through
>>Mostly I am mounting immunolabelled frozen tissue sections with DAB. I
>>know aqueous mountants are generally used for AEC but I dont see the
>>point in doing the alcohol and xylene steps if an aqueous mountant
>>works. I dont do paraffin sections so dont already have this set-up.
>>Vector say that their aqueous mountant is also suitable for DAB but is
>>this true for all aqueous mountants? I've been looking at Faramount
>>Aqueous mountant from Dako - any comments?
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