[Histonet] Giemsa as counterstain

Martin, Erin Erin.Martin <@t> ucsf.edu
Tue Apr 24 11:11:16 CDT 2007

Hello all,
Have any of you every used giemsa as an IHC counterstain for melanin containing tissues?  It  apparently turns the melanin green which differentiates it from the DAB.  My docs have seen this and want to use it here.  I bought giemsa solution from American Master Tech and tried it, but the docs said it doesn't look right.  If you have used giemsa for this purpose or know what I am doing wrong, I would appreciate any input!
Thanks so much,
Erin Martin
San Francisco

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