[Histonet] Re: Aqueous mountant suggestions please

[Gayle Callis]

The Faramount works, but it must be heated before coverglass application, 
and the heating adds another step you have to deal with, Howevere, it is 
very nice aqueous media that sets up hard and clear.

We have also done frozen sections with DAB and set up the 
dehydration/clearing for permanent mounting media.  I must admit this gives 
a superb final preparation that when dry, the mounting media doesn't 
retract under the coverglass.  It is not that difficult to set up the 
dehyration/clearing/permanent mountant,  and there are some nice 
mini-staining devices to help you out depending on how many slides you 
stain at one time.   We prefer dehydration/clearing/and permanent media 
with DAB.



At 09:39 AM 4/24/2007, you wrote:
>Quick query,
>
>I need and aqueous mountant for immunohistochemistry slides. I am
>currently using Vectamount AQ from Vectashield but am having some
>problems with it not hardening completely and it doesnt seem to last
>very long ie. the use by date is already up and I'm only halfway through
>the bottle.
>
>Mostly I am mounting immunolabelled frozen tissue sections with DAB. I
>know aqueous mountants are generally used for AEC but I dont see the
>point in doing the alcohol and xylene steps if an aqueous mountant
>works. I dont do paraffin sections so dont already have this set-up.
>
>Vector say that their aqueous mountant is also suitable for DAB but is
>this true for all aqueous mountants? I've been looking at Faramount
>Aqueous mountant from Dako - any comments?
>
>Thanks
>Sonya
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610