[Histonet] Aqueous mountant suggestions please

[Horn, Hazel V]

We use Faramount from DAKO.   The docs like it.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3912

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin
S.
Sent: Tuesday, April 24, 2007 10:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Aqueous mountant suggestions please

Quick query,
 
I need and aqueous mountant for immunohistochemistry slides. I am
currently using Vectamount AQ from Vectashield but am having some
problems with it not hardening completely and it doesnt seem to last
very long ie. the use by date is already up and I'm only halfway through
the bottle.
 
Mostly I am mounting immunolabelled frozen tissue sections with DAB. I
know aqueous mountants are generally used for AEC but I dont see the
point in doing the alcohol and xylene steps if an aqueous mountant
works. I dont do paraffin sections so dont already have this set-up.
 
Vector say that their aqueous mountant is also suitable for DAB but is
this true for all aqueous mountants? I've been looking at Faramount
Aqueous mountant from Dako - any comments?
 
Thanks
Sonya


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