[Histonet] alcian blue_ precipitation

[Akemi Allison-Tacha]

This is what I do with my Alcian Blue.  My stabalizer
is missing from this protocol because it is
proprietary.  By the way, Anatech has the best Alcian
Blue on the market! The most dye content!!!

1.	Label the appropriately sized container with
description, lot number, storage conditions,
expiration date, prepared by and date.  Add a large
stir bar to the container.

2.	Measure the 3% acetic acid solution determined in
the Calculation section of Manufacturing Document. 
Transfer the 3% Acetic Acid to the labeled container,
and begin stirring on a large stir plate.  Add the
Alcian Blue and continue mixing solution.  

3.	Mix thoroughly for 30±5 minutes or until all the
chemicals and reagents have dissolved.  Visually
inspect solution to ensure adequate mixing.

4.	Measure the pH of the solution using a pH meter
calibrated at pH 1 and pH 4.  The pH must be 2.5 ±
0.1.  If necessary, adjust pH with NaOH of HCL.  

5.	The reagent is stable for 12 months at room
temperature.  Store the reagent at room temperature in
an opaque container.

6.	Test for quality control. 

Good Luck,
Akemi Allison-Tacha

--- raghul <raghulvet <@t> yahoo.co.in> wrote:

> dear histonetters
>    
>   When i try to dissolve the alcian blue (sigma)
> powder in acidified (ph2.5/ph1.0) dd water the stain
> isnt getting dissolved. When i try 1:1
> alchol:distilled water adjusted to required pH its
> dissolving but isnt staining any of mucin cells in
> positive control. please help
>    
>   raghul
>   Medbiotech res. limited
>   chennai, India
> 
>        
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