[Histonet] alcian blue_ precipitation

[raghul]

dear histonetters
   
  When i try to dissolve the alcian blue (sigma) powder in acidified (ph2.5/ph1.0) dd water the stain isnt getting dissolved. When i try 1:1 alchol:distilled water adjusted to required pH its dissolving but isnt staining any of mucin cells in positive control. please help
   
  raghul
  Medbiotech res. limited
  chennai, India

       
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