[Histonet] alcian blue_ precipitation

bhewlett <@t> cogeco.ca bhewlett <@t> cogeco.ca
Tue Apr 17 09:08:55 CDT 2007

> Raghul,

try dissolving the Alcian blue powder in distilled water first, then adjust the pH with acid. You should have no problem
with pH 2.5, but some lot #s of dye will precipitate at pH 1.0. If this happens contact me again.


> dear histonetters
>   When i try to dissolve the alcian blue (sigma) powder in acidified (ph2.5/ph1.0) dd water the stain isnt getting
> dissolved. When i try 1:1 alchol:distilled water adjusted to required pH its dissolving but isnt staining any of mucin
> cells in positive control. please help
>   raghul
>   Medbiotech res. limited
>   chennai, India
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