[Histonet] Zinc Formalin and Iron stains

[Tarango, Mark]

I don't know, we only use 10% NBF for bone marrows here, but I've seen a
video of some cells getting fixed with zinc formalin.  The cells were
popping and exploding all over the place.  Made me think, "no wonder you
get that great nuclear detail."




Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV  89135

mtarango <@t> nvcancer.org

Direct Line (702) 822-5112

Fax (702) 939-7663

  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hannen,
Valerie
Sent: Monday, April 16, 2007 11:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Zinc Formalin and Iron stains

Hi fellow histonetters!!
 
    We are just wondering if anyone else has run into this problem...we
have been told by our Pathologist that smears that we make from a
Bone Marrow aspirate (before fixation) show positive Iron staining...
however, the clot from the same aspirate that has been fixed in
Zinc Formalin showed no Iron staining. Has Zinc Formalin been
known to leach Iron out of Bone Marrow aspirates?
Any insight that you could share with us would be greatly 
appreciated.
                             
                                   Valerie Hannen,MLT(ASCP),HTL,SU(FL)
                                   Parrish Medical Center
                                   Titusville, Florida

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