[Histonet] derm path

Richard Cartun Rcartun <@t> harthosp.org
Mon Apr 16 09:30:35 CDT 2007

I think the more important issues here are 1.) how much experience do you have doing immunohistochemical (IHC) staining and 2.) what will be your volume as you move forward.  Keep in mind that many DermPath diagnoses are based on IHC findings and you need to perform validations on-site (for every primary antibody) to demonstrate that you are obtaining the correct IHC results.  Are you prepared to deal with issues concerning fixation and tissue processing, antigen retrieval, nonspecific staining, antibody cross-reactivity, appropriate negative and positive controls, etc.?  In my opinion, you should not be doing IHC unless you are performing the tests on a regular basis and you have an experienced staff  to do the job right, including a pathologist to assist and support you.


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> <godsgalnow <@t> aol.com> 04/16/07 9:35 AM >>>
Hello all,
We are thinking of adding another client, but it is a derm client.  Can anybody tell me what all antidoies I will need to have on hand?  I know the basics: Melan A, Pan Keratin, S100, HMB45, etc--but what else may they ask for? 
Thanks in advance,
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