[Histonet] HSV 1&2 staining

[Barry Madigan]

Hi Jim,

We prepare a antibody cocktail of HSV I & II both from DAKO.
HSV1 1:400
HSV2 1:800
No pre-treatment and 15 minute incubation in the antibody cocktail.
No heat
On a Vision BioSystem BONDMAX using the Polymer Define Detection System.
I am certain it will work just as well on the DAKO Autostainer.

Regards
Barry Madigan HT (ADCLT)
Immunohistochemistry
QHPS-RBH
Royal Brisbane Hospital
Australia


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
jimr0712 <@t> comcast.net
Sent: Saturday, 14 April 2007 1:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HSV 1&2 staining

Could someone share the current methodology that they are using on the DAKO
Autostainer Plus for HSV 1 & 2 staining ?  Thanks.

--
Jim Robinson, M.S., HTL/HT (ASCP)
Director of Laboratory Operations
Orizon Diagnostics, LLC
102 Chestnut Avenue
Westmont, Illinois 60559
jrobinson <@t> orizondiagnostics.com
630-321-1506 (fax)
630-230-6325

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