[Histonet] Fluorescein Question

Breeden, Sara sbreeden <@t> nmda.nmsu.edu
Fri Apr 13 09:48:01 CDT 2007

A current project is cutting slides to check for fluorescein expression.
The first time I did this, I was asked to air-dry the slides and mount
with aqueous medium; as you can expect, the coverage was "iffy" at best
with lifting of the coverslip, etc.  Is there any reason I could not dry
these in a 60 degree oven as usual, then deparaffinize and coverslip out
of xylene?  Would this process compromise the results? Help? Thanks!


Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 700

Albuquerque, NM  87106



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