[Histonet] rodent smooth muscle marker

[Liz Chlipala]

What about alpha smooth muscle actin, most anti-human SMA antibodies will
cross react with rodent.  If she is planning on both rat and mouse tissues
then I would choose a rabbit antibody to smooth muscle actin.  The one that
we use works in both rat and mouse, its from abcam ab5694.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jacqui
Detmar
Sent: Thursday, April 12, 2007 7:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] rodent smooth muscle marker

Hi all.  I am asking this question on behalf of a colleague.  She wishes to
measure rat uterine smooth muscle cell volume over gestation and would like
to have a marker that would delinate the smooth muscle plasma membrane.  I
have looked at some of my Masson trichrome-stained mouse placental sections
and this *might* be helpful for her; however, I was thinking one of you
might know of a good lectin or antibody marker.  Like I said, she works on
rat tissue, but I wouldn't mind receiving some advice on mouse uterine
smooth muscle cell markers, too.
 
Thanks a bunch!
 
Jacqui Detmar
Samuel Lunenfeld Research Institute, room 876
Mount Sinai Hospital
600 University Avenue
Toronto, ON   M5G 1X5
 
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