[Histonet] (no subject)

[Joe Nocito]

Sally,
I'm using a Pyrex beaker and changing the distilled water each time. I set 
my times by the times used for the special stains. For instance, my lowest 
time in the microwave is 15 seconds, my highest time is 45 seconds. I set up 
a chart with 15, 30, and 45 seconds. I run this three times for each time 
and get an average.
    When I had my inspection in January, the inspector took a copy of my 
procedure and chart with her. Again, each inspector is different, but it 
passed. I'd be happy to share and I promise to keep my toes to myself.

JTT
----- Original Message ----- 
From: "Norton, Sally" <sally.norton <@t> seattlechildrens.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, April 06, 2007 3:38 PM
Subject: [Histonet] (no subject)


            Hi All,



            We are wondering how others are testing temperature
reproducibility on their microwaves.  We are using a plastic coplin jar
filled with distilled water.  We then heat it at different powers and
times and take the temperature with a thermometer.  Any suggestions for
a better way?



            Thank you.



            Sally Norton

            Histology

            Seattle Children's Hospital and Regional Medical Center


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