AW: [Histonet] re: why do we use xylene? was - Peloris Processor and

Gudrun Lang gu.lang <@t>
Fri Apr 6 13:09:02 CDT 2007

With a look in the isopropanol-datasheet it is to be seen, that this reagens
is not really "safe". It has a flashpoint at 12°C. Perhaps not the ideal
reagens to be used in electric instruments with hot temperatures?

That's in my opinion the cause, why it wasn't generally used in the over-all
VIPs. New developments could offer the necessary safety?

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Carl Hobbs
Gesendet: Freitag, 06. April 2007 19:47
An: Histonet
Betreff: [Histonet] re: why do we use xylene? was - Peloris Processor and

In the late 70s I trialled isopropanol (IPA) as a processing 
dehydrant/"clearing" agent. It was completely successful for me for all my 
routine tinctorial/histochemical staining ( I wasn't using Immuno at that 
time, so cannot comment on that area).
IPA is NOT miscible with wax at RT ( unlike xylene) but, at the temp. 
neccessary to melt your conventional waxes ( at least 60C) it is completely 
miscible. I mentioned this, I think. I stopped using it as I 
subsequently took a lower post in Clinical labs and I was laughed at  when I

suggested testing IPA out.
Great to read that it's come up again: perhaps I had also read  Romeis' 
article in a journal, before it was published in a book?
Too long ago to remember, lol.


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