AW: [Histonet] re: why do we use xylene? was - Peloris Processor and
gu.lang <@t> gmx.at
Fri Apr 6 13:09:02 CDT 2007
With a look in the isopropanol-datasheet it is to be seen, that this reagens
is not really "safe". It has a flashpoint at 12°C. Perhaps not the ideal
reagens to be used in electric instruments with hot temperatures?
That's in my opinion the cause, why it wasn't generally used in the over-all
VIPs. New developments could offer the necessary safety?
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Carl Hobbs
Gesendet: Freitag, 06. April 2007 19:47
Betreff: [Histonet] re: why do we use xylene? was - Peloris Processor and
In the late 70s I trialled isopropanol (IPA) as a processing
dehydrant/"clearing" agent. It was completely successful for me for all my
routine tinctorial/histochemical staining ( I wasn't using Immuno at that
time, so cannot comment on that area).
IPA is NOT miscible with wax at RT ( unlike xylene) but, at the temp.
neccessary to melt your conventional waxes ( at least 60C) it is completely
miscible. I mentioned this before...here, I think. I stopped using it as I
subsequently took a lower post in Clinical labs and I was laughed at when I
suggested testing IPA out.
Great to read that it's come up again: perhaps I had also read Romeis'
article in a journal, before it was published in a book?
Too long ago to remember, lol.
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
More information about the Histonet