[Histonet] re: why do we use xylene? was - Peloris Processor and
carl.hobbs <@t> kcl.ac.uk
Fri Apr 6 12:47:18 CDT 2007
In the late 70s I trialled isopropanol (IPA) as a processing
dehydrant/"clearing" agent. It was completely successful for me for all my
routine tinctorial/histochemical staining ( I wasn't using Immuno at that
time, so cannot comment on that area).
IPA is NOT miscible with wax at RT ( unlike xylene) but, at the temp.
neccessary to melt your conventional waxes ( at least 60C) it is completely
miscible. I mentioned this before...here, I think. I stopped using it as I
subsequently took a lower post in Clinical labs and I was laughed at when I
suggested testing IPA out.
Great to read that it's come up again: perhaps I had also read Romeis'
article in a journal, before it was published in a book?
Too long ago to remember, lol.
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