[Histonet] double-staining with two rabbit antibodies, Zenon-kit

Jimmy Lao lao_ji <@t> yahoo.com
Thu Apr 5 09:37:34 CDT 2007

Can you tell me how to do the "block well" before
implementing the second (rabbit primary#2) set Ab
Thanks in advance!

--- AGrobe2555 <@t> aol.com wrote:

> Yves,
> Just do the complete stain for one (block, rabbit
> primary#1, fluorescent  
> secondary#1), block well and then do the second
> (rabbit primary#2, fluorescent  
> secondary#2).  Use two different color anti-rabbit
> fluorophores.  I  have done 
> this in the past with no apparent problems.    
> Another option is to pre-react the two primaries
> with different anti-rabbit  
> fluorophores in separate tubes, and then add to the
> section. 
> Albert
> Albert C.  Grobe, PhD
> International Heart Institute of Montana Foundation
> Tissue  Engineering Lab, Saint Patrick Hospital
> ************************************** See what's
> free at http://www.aol.com.
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