[Histonet] double-staining with two rabbit antibodies, Zenon-kit

AGrobe2555 <@t> aol.com AGrobe2555 <@t> aol.com
Tue Apr 3 14:05:18 CDT 2007

Just do the complete stain for one (block, rabbit primary#1, fluorescent  
secondary#1), block well and then do the second (rabbit primary#2, fluorescent  
secondary#2).  Use two different color anti-rabbit fluorophores.  I  have done 
this in the past with no apparent problems.    
Another option is to pre-react the two primaries with different anti-rabbit  
fluorophores in separate tubes, and then add to the section. 
Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick Hospital

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