[Histonet] Myosin Heavy Chain (slow) Antibodies

[Dana Settembre]

Hello Rhonda,
I use NovoCastra's Myosin Heavy Chain (slow) on FFPE human tissue.
The spec sheet indicates that this antibody reacts with mouse among
others.
NovoCastra is distributed in by Vision BioSystems in
Norwell, MA  800-753-7264
The cat. # is: NCL-MHCs

I use normal muscle as positive control.
I pretreat with Dako's Target Retrieval Sol'n (TRS) in a steamer for 40
min.
(but I have pretreated other ways and they work too)
I use it at a 1:40 dilution.  It looks good (on human tissue)

Good Luck




Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> "Rogers, Rhonda" <rhrogers <@t> iupui.edu> 04/04/07 6:31 PM >>>
Hello,

 

I am looking for a myosin heavy chain (slow) antibody that will work
on
FFPE sections.  I will be using mouse embryo sections.  If anyone is
willing to share recommendations or protocols, I would appreciate some
help.

 

Thanks,

 

Rhonda

 

Rhonda Rogers, HTL(ASCP)

Wells Center for Pediatric Research - Conway Laboratory

Indiana University Purdue University Indianapolis

1044 W. Walnut St., R4 379

Indianapolis, IN  46202-5225

 

Phone: 317-278-8781     E-mail: rhrogers <@t> iupui.edu 

 

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