AW: [Histonet] Peloris Processor and isopropanol

[Gudrun Lang]

I've seen a protocol for isopropanol-processing with an IPA-temperature
about 74 degrees. That is near the boilingpoint and means rather a boiling
out of the tissue than acting as intermedium. Similar protocols are found
with the microwave-techniques. I think there is no principal difference in
reaching the temperature.

What I'm a little confused about is the fact, that usually recommended
temperatures for tissueprocessing don't raise above 62 degrees. So how does
this influence staining, ihc-staining etc.?
What are the temperatures in your protocol?
What does the community think about this issue?

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Judy
Collins
Gesendet: Montag, 02. April 2007 15:53
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Peloris Processor

We have the Peloris rapid tissue processor.  It is xylene free but does
not use microwaves.  It uses isopropyl alcohol after the reagent alcohol
to remove all traces of water and then goes straight to the paraffin
>from there.  Amazingly, and contrary to everything we have been taught,
this works!

It processes small biopsies in approximately 1 hour.  Shave biopsies
take approximately 2 hours and the larger specimens 4 to 6 hours.  We
find the processing to be at least equal to routine processing and in
many cases, superior to it.  Because it does not use xylene, the tissues
are not hard and brittle as they can sometimes be with routine
processing.

It has two separate retorts which can run two separate runs at the same
time.  We are able to process our specimens throughout the day instead
of everything being processed at night as in the past. It has greatly
improved our TAT.

The Peloris tracks the number of blocks run and, based on this, tells
you when to change the various solutions.  As a result, we have saved a
significant amount of money on reagents. 

We are extremely happy with ours.


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