[Histonet] Re: effects of elevation on immunohistochemistry

[Gayle Callis]

Lisa,

At 4800 ft above sea level, nothing on actual staining for application of 
reagents.   However, it may affect your HIER or heated retrievals since 
water boils at a lower temperature at high altitude.  I would assume one 
might have to establish retrieval time if deemed a problem. Altitude was 
discussed on Hitonet many years ago (check archives). Patsy Ruegg, who 
works at an even higher elevation in Colorado addressed this point.  You 
should also ask the Ventana tech services too - some of them came from 
Flagstaff AZ, which is REALLY high altitude and their company is in 
Arizona.  Hopefully Liz Chlipala will address this issue since she is in 
Colorado.

I never do retrievals, but my staining protocols without problems.

At 08:21 AM 9/28/2006, you wrote:
>Good morning,
>   Does anyone have any experience or knowledge about the  effects of 
> elevation on immunohistochemistry?
>   Thanks kindly, Lisa Manning

Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717