[Histonet] Decal for Immunos

Tony Henwood AnthonyH <@t> chw.edu.au
Tue Sep 26 18:06:50 CDT 2006

The following might be of use:

Organic acids are slower decalcifiers and are usually gentler on

Formic acid (5%) has been extensively used primarily as a fixative but
has good decalcifying properties especially for bone marrow trephine
biopsies. Picric acid is rarely used by itself but is usually used in
combination with other acids or fixatives. Acetic acid tends to shrink
tissues so is usually combined with other ingredients. Trichloroacetic
acid (4%) is very energetic in its action but tends to deteriorate in

Foschini & Muzzi (1993) described the use of 7% citric acid for the
decalcification of small calcifications such as psammoma bodies. These
small calcifications can render tissues difficult to cut and often the
tissue surrounding the concretion is shattered and disappears
microscopically. Though this gentle decalcification is not appropriate
for dense bones such as teeth and femurs, it is quite adequate for the
small calcifications (no greater than 1-2mm) where overnight treatment
is enough.

EDTA solutions are also quite usefull


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
Sent: Wednesday, 27 September 2006 2:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Decal for Immunos

Hi everyone,

Does anyone know of a decal solution without HCL?  It seems our RapidCal
interfering with some of the immuno stains.

Thanks so much!
Paula Wilder
St. Joseph Medical Center
Towson, MD 21204

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