[Histonet] Antigen retrieval in the microwave

Phil McArdle pmcardle <@t> ebsciences.com
Tue Sep 26 15:44:35 CDT 2006

If you'll forgive a vendor for butting in...

Regarding the microwave pressure cooker: Let's not confuse "microwave 
transparency" with what we typically associate with the concept of 
transparency: /optical/ transparency. They're not the same. Microwave 
transparency refers to an object's ability to allow passage of microwave 
energy without converting a significant portion to heat. While some 
optically transparent objects happen also to be microwave transparent, 
that's not always true; leaded crystal in a microwave would be a 
disaster. Conversely, opaque white Teflon isn't something we'd think of 
as "transparent," yet it IS "microwave transparent."

EBS has just produced a "Microwave Companion for Histology," a 20-page 
booklet that seeks to put to rest just this kind of confusion, and it's 
free for the asking. It's purely informational, not looking to sell 
anything, with a view towards "advancing the art" rather than "shameless 
commerce." Please contact me directly and I'll be happy to send you one, 
or a few.

Best regards,

Phil McArdle

Microwave Product Manager
Energy Beam Sciences, Inc.
Tel:  800.992.9037 x 341
Fax: 860.653.0422
PMcardle <@t> ebsciences.com

Terri Braud wrote:
> The trick will be to only use transparent containers, another CAPmania reg...my microwave pressure cooker just got 86'd.  I would not consider citrate buffer to be Hazardous.....good grief, I have boiled salt water in mine at home, and have lived to tell the tale.  Terri
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supv.
> Laboratory
> Holy Redeemer Hospital
> (215) 938-3689
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Angela
> Bitting
> Sent: Tuesday, September 26, 2006 2:24 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Antigen retrieval in the microwave
> I want to make sure I'm, totally CAP compliant, soooooo here's my
> question.
> Has anyone gotten cited for heating their citrate buffer or EDTA (for
> antigen retrieval)  in an unvented microwave? 
> I'm getting the impression that CAP wants all microwaves vented even
> though they throw out that blurb about "unnecessary if only
> non-hazardous solutions are being heated, i.e. water, some biological
> stains, paraffin sections".
> Angela Bitting, HT(ASCP)
> Technical Specialist, Histology
> Geisinger Medical Center 
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone  570-214-9634
> fax  570-271-5916
> IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
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