[Histonet] Antigen retrieval in the microwave

Terri Braud tbraud <@t> holyredeemer.com
Tue Sep 26 14:21:22 CDT 2006

The trick will be to only use transparent containers, another CAPmania reg...my microwave pressure cooker just got 86'd.  I would not consider citrate buffer to be Hazardous.....good grief, I have boiled salt water in mine at home, and have lived to tell the tale.  Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supv.
Holy Redeemer Hospital
(215) 938-3689

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Angela
Sent: Tuesday, September 26, 2006 2:24 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Antigen retrieval in the microwave

I want to make sure I'm, totally CAP compliant, soooooo here's my
Has anyone gotten cited for heating their citrate buffer or EDTA (for
antigen retrieval)  in an unvented microwave? 
I'm getting the impression that CAP wants all microwaves vented even
though they throw out that blurb about "unnecessary if only
non-hazardous solutions are being heated, i.e. water, some biological
stains, paraffin sections".

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916

IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu


This E-Mail is intended only for the use of the individual or entity to which 
it is addressed. It may contain information that is privileged and/or confidential, 
and the use or disclosure of such information may also be restricted under applicable 
federal and state law. If you received this communication in error, please do not 
distribute any part of it or retain any copies, and delete the original E-Mail.
Please notify the sender of any error by E-Mail at the electronic address shown.

Thank you for your cooperation.

More information about the Histonet mailing list