[Histonet] Antigen retrieval in the microwave

[Terri Braud]

The trick will be to only use transparent containers, another CAPmania reg...my microwave pressure cooker just got 86'd.  I would not consider citrate buffer to be Hazardous.....good grief, I have boiled salt water in mine at home, and have lived to tell the tale.  Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supv.
Laboratory
Holy Redeemer Hospital
(215) 938-3689


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Angela
Bitting
Sent: Tuesday, September 26, 2006 2:24 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Antigen retrieval in the microwave


I want to make sure I'm, totally CAP compliant, soooooo here's my
question.
Has anyone gotten cited for heating their citrate buffer or EDTA (for
antigen retrieval)  in an unvented microwave? 
I'm getting the impression that CAP wants all microwaves vented even
though they throw out that blurb about "unnecessary if only
non-hazardous solutions are being heated, i.e. water, some biological
stains, paraffin sections".



Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916


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