[Histonet] Antigen retrieval in the microwave

Rene J Buesa rjbuesa <@t> yahoo.com
Tue Sep 26 13:31:08 CDT 2006

Nobody that I know off. Citrate buffer falls into the classification of non-hazardous solutions (0.1 M conc. solutions).
  René J.

Angela Bitting <akbitting <@t> geisinger.edu> wrote:
  I want to make sure I'm, totally CAP compliant, soooooo here's my
Has anyone gotten cited for heating their citrate buffer or EDTA (for
antigen retrieval) in an unvented microwave? 
I'm getting the impression that CAP wants all microwaves vented even
though they throw out that blurb about "unnecessary if only
non-hazardous solutions are being heated, i.e. water, some biological
stains, paraffin sections".

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916

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