[Histonet] mounting fluorescent tissue

Markus F. Meyenhofer micro <@t> superlink.net
Thu Sep 21 21:31:42 CDT 2006

a little light won't do any harm!
Just think of the high intensity of UV light you will bombard your samples 
under the microscope !!!!
Markus F. Meyenhofer
----- Original Message ----- 
From: "Caroline Bass" <cbass <@t> bidmc.harvard.edu>
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, September 21, 2006 10:19 AM
Subject: [Histonet] mounting fluorescent tissue

> Hi, I have what might be a silly question.  I am dealing with a lot  of 
> native fluorescence, particularly in the brain, 40 um floating  sections. 
> At some point I want to mount the sections to observe the  native 
> fluorescence.  I have been doing this in a dimly lit room, but  it can be 
> difficult at times.  Do you think there would be a loss of  signal if I 
> mount the sections under normal light conditions?  Also,  if I choose to 
> do immunofluorescence should I be careful, I heard  that FITC staining 
> should be done in the dark and I assume the  mounting should as well.
> Any advice is appreciated.  Thanks, Caroline
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