[Histonet] Yeh glad to be back & ??

[Amy Porter]

Is anyone out there doing Tunel on FFPE acid decalcifed sections.  I am trying to stain for apoptotic cells using a Tunel kit on decalcified mouse femurs.  Not having much success, if anyone has any tips or do's / don'ts I would really appreciate the information.  Thanks in advance, 


Amy S. Porter, HT(ASCP) QIHC - Supervisor
Michigan State University
Investigative HistoPathology Laboratory
Department of Physiology / Division of Human Pathology
2100 Biomedical Physical Sciences Bldg. Room #2133
East Lansing, MI  48824-3320
Phone:  (517) 355-6475 ext 1480 / Fax:  (517) 432-1368
Email:  portera <@t> msu.edu
www.humanpathology.msu.edu