[Histonet] tissues in cleaning cycle

[Timothy Macatee]

Is the Histonet down, no messages today.  Or did I quit accidently?

Tim


On 9/6/06 12:23 PM, "mari.ann.mailhiot <@t> leica-microsystems.com"
<mari.ann.mailhiot <@t> leica-microsystems.com> wrote:

> Renee
> 
> As long as the last step on the cleaning cycle is 100% alcohol not water
> you can go to xylene and paraffin. But usually cleaning cycles consist of
> xylene, 100% alcohol, and water. Water being the last step.
> 
> Also some cleaning cycleces use heat around 60 degrees. Do you want your
> specimens subjected to the heat for the amount of time the cleaning cycle
> runs? It may be something to think about.
> 
> I don't know what processor you have but you may be able to creat a program
> that starts with an alcohol like 95%. You may want to check with your
> processor rep on whether you can try and do it.
> 
> Hope this helps.
> 
> Mari Ann Mailhiot BA HT ASCP
> Application Specialist
> Leica Technical Assistance Center
> 800 248 0123 x7267
> 847 236 3063 fax
> mari.ann.mailhiot <@t> leica-microsystems.com
> www.leica-microsystems.com
> 
> 
>                  
>              "Till, Renee"
>              <TillRenee <@t> uams.e
>              du>                                                        To
>              Sent by:                  histonet <@t> lists.utsouthwestern.edu
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>              lists.utsouthwest
>              ern.edu                                               Subject
>                                        [Histonet] tissues in cleaning
>                                        cycle
>              09/06/2006 07:49
>              AM  
>                  
>                  
>                  
>                  
> 
> 
> 
> Hello. Any suggestions on what to do with tissues that were accidentally
> left in the processor during the cleaning cycle? I would think they need
> to be re-infiltrated with paraffin at the very least. I don't know if
> our processor will let us do anything but a full processing run.
> 
> 
> 
> Renee' Till, HT
> 
> Research Assistant
> 
> Arkansas Children's Nutrition Center
> 
> 1212 Marshall St./N2021
> 
> Little Rock, AR 72002
> 
> Office (501)364-2785
> 
> Fax (501)364-3161
> 
> 
> 
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-- 
Tim Macatee
Research Histology Core
New York University School of Medicine
550 First Ave.
Department of Pathology.
Medical Science Building - Room 521
New York, N.Y. 10016
(212) 263-3888