[Histonet] tissues in cleaning cycle

[mari.ann.mailhiot <@t> leica-microsystems.com]

Renee

As long as the last step on the cleaning cycle is 100% alcohol not water
you can go to xylene and paraffin. But usually cleaning cycles consist of
xylene, 100% alcohol, and water. Water being the last step.

Also some cleaning cycleces use heat around 60 degrees. Do you want your
specimens subjected to the heat for the amount of time the cleaning cycle
runs? It may be something to think about.

I don't know what processor you have but you may be able to creat a program
that starts with an alcohol like 95%. You may want to check with your
processor rep on whether you can try and do it.

Hope this helps.

Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com


                                                                           
             "Till, Renee"                                                 
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                                       [Histonet] tissues in cleaning      
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             09/06/2006 07:49                                              
             AM                                                            
                                                                           
                                                                           
                                                                           
                                                                           



Hello. Any suggestions on what to do with tissues that were accidentally
left in the processor during the cleaning cycle? I would think they need
to be re-infiltrated with paraffin at the very least. I don't know if
our processor will let us do anything but a full processing run.



Renee' Till, HT

Research Assistant

Arkansas Children's Nutrition Center

1212 Marshall St./N2021

Little Rock, AR 72002

Office (501)364-2785

Fax (501)364-3161




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