[Histonet] Alternative to DAPI

Mathew DeGutes m-degutes <@t> northwestern.edu
Thu Sep 14 13:57:55 CDT 2006

Are there any alternative nuclear fluorescent stains to DAPI that I can use in the Blue (455-495)
spectrum of excitation that will work with frozen sections that are very quickly acetone fixed?  I
am trying to utilize this for LCM RNA extraction so time is of course a VERY important factor as
well.  My other fluorophore would be Alexa-555 so I don't want the two to overlap.

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