[Histonet] BrdU control

[Tarango, Mark]

Wouldn't you just use the tissue that you have for the project as the
control?  If you injected BrdU into the animal before you sacrificed it,
then it should stain positive (provided the tissue has dividing cells in
it), so I'm thinking that you should be able to use the tissue for the
project as the positive control and tissue from an animal not treated
with BrdU as the negative control.  Right?



Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV  89135

mtarango <@t> nvcancer.org

Direct Line (702) 822-5112

Fax (702) 939-7663

  


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Histology Field
Sent: Tuesday, September 12, 2006 12:18 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] BrdU control

Hi there,
   
  I was wondering if anyone out there had any BrdU control tissue that
they would be willing to share?  I am working on a project that requires
BrdU staining, but unfortunately, I wasn't given any control tissue.
Baboon gut BrdU tissue would be great?   Or any other control tissue
from any species.   Any help would be appreciated!
   
  Thanks!
  N

 		
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"MMS <nvcancer.org>" made the following annotations.
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