[Histonet] Control tissue for skin DIF

Chris Pomajzl cpomajzl <@t> cpllabs.com
Mon Sep 11 07:24:42 CDT 2006

We use fresh tonsil for +CTRL. We cut a tonsil down into many tiny pieces
and freeze them in OCT. When doing DIF on skin, we will thaw one piece of
tonsil and embed it in the same chuck as the skin specimen so that both
specimens are on the same slide. It has worked very well since we started
doing this. The pathologists love it.

----- Original Message ----- 
From: "Luke A Perkocha" <luke.perkocha <@t> ucsf.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, September 08, 2006 8:42 AM
Subject: [Histonet] Control tissue for skin DIF

>    Hello,
>    I  am trying to develop a protocol for doing direct immunofluorescence
>    testing  on  skin  for  bullous  disease.  I  have  a couple of sample
>    protocols  but  none  say  anything about positive control tissue. I'm
>    uncomfortable running the test on clinical specimens and reporting out
>    a  negative,  without  good  positive  controls. Does anyone know of a
>    commercial  source  for  positive  control tissue, or does anyone have
>    some  they  would  be  willing  to  share  for  development? Help much
>    appreciated.
>    Thanks,
>    Luke
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