[Histonet] Control tissue for skin DIF
Joe Nocito
jnocito <@t> satx.rr.com
Fri Sep 8 20:21:52 CDT 2006
Luke,
if you find a source, please let me know. We save cases that stain
positive, but we don't have a full spectrum of known controls. Our dermpath
is content with that (no, really, this is a huge, huge point, this lady is
not content with anything else). I've contacted several sources. They do not
or will not commit to storing frozen section slides and still guarantee
their antigenicity, which I can't blame them because our own controls fade
after a while.
I know this wasn't that helpful, but hell, it's Friday, so let's enjoy
the weekend.
Joe Nocito BS, HT(ASCP)QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
----- Original Message -----
From: "Luke A Perkocha" <luke.perkocha <@t> ucsf.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, September 08, 2006 8:42 AM
Subject: [Histonet] Control tissue for skin DIF
>
> Hello,
> I am trying to develop a protocol for doing direct immunofluorescence
> testing on skin for bullous disease. I have a couple of sample
> protocols but none say anything about positive control tissue. I'm
> uncomfortable running the test on clinical specimens and reporting out
> a negative, without good positive controls. Does anyone know of a
> commercial source for positive control tissue, or does anyone have
> some they would be willing to share for development? Help much
> appreciated.
> Thanks,
> Luke
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