[Histonet] CAP Question

[Sebree Linda A.]

Laurie,

We have a statement in our Laboratory Procedure Manual under "Antibody
Optimization" that reads:  "The selection of avidin biotin (AB) blocking
will be dependent on the specimen source.  Examples of tissues
benefiting from AB block include kidney and liver.  It may be necessary
to have more than one protocol programmed to offer the choice of
blocking or not."

Hope this helps,

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Tuesday, September 05, 2006 4:15 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CAP Question


Can anyone help explain what kind of policy I would need for the
following CAP question:

ANP.22615:
If the laboratory uses an avidin-biotin complex (ABC) detection system
(or a related system such as streptavidin-biotin or neutravidin-bioton),
is there a policy that addresses nonspecific false positive staining
from endogenous bioton?

Laurie Colbert
Huntington Hospital

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