[Histonet] more rigid paraffin

[gillian.2.brown <@t> gsk.com]

Patsy,
You need to use a GMA procedure where you start off fixing in ice-cold 
acetone and the whole infiltration procedure is carried out at 4 degrees, 
rather than a 'standard GMA method.
Brilliant morphology and retained antigenicity and as desired little 
distortion between sections.
However size can be a limitation, 2mm is recommended but I've done whole 
mouse heads.
Look out  papers by Susan Wilson or I could send you our protocol (which 
is hers).

Cheers
Gill Brown




GlaxoSmithKline Medicines Research Centre,
Gunnelswood Road,
STEVENAGE,
Hertfordshire.
  SG1 2NY





"Patsy Ruegg" <pruegg <@t> ihctech.net> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05-Sep-2006 20:48
 
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Subject
[Histonet] more rigid paraffin






Does anyone know of a paraffin which will stretch less/distort less than
most?  We are trying to digitally reconstruct tissue 3 dimensionally at 
the
histology level by scanning tissue sections completely thru the sample and
line them up one after the other but the paraffin sections stretch and
distort so that one section does not match it's deeper counter part.  We
thought if we had a stiffer medium this distortion from section to deeper
section would be reduced?  We are thinking about using GMa instead 
paraffin
to embed expecting less distortion but we also want to do IHC and that is
problematic with GMA.  Any suggestions for this project would be
appreciated.

Thanks,

Patsy Ruegg

IHCtech

12635 Montview Blvd. Ste.215

Aurora, Colorado 80045

Phone: 720-859-4060

Fax: 720-859-4110

pruegg <@t> ihctech.net

www.ihctech.net 

 

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