[Histonet] melanin pigment

[Lori Richey]

We use DAB and do a giemsa counterstain.  The melanin pigment turns a 
dark green with the giemsa.

Perry, Margaret wrote:

>Mel wrote: 
>
>I don't recall this question, but I would be interested in how you get
>around the problem of epidermal pigmentation if you are looking for
>protein expression in the epidermis; do you bleach the tissue (and if
>so, does that affect antigenicity of the proteins), or can you choose a
>detection chromogen that has a wild color, like a Barney purple color? 
>
>Thanks for your help.
>
>Mel
>
> 
>
>We use Vectors Nova Red and it works well with melanin and also
>hemosiderin.
>
> 
>
>Margaret Perry HT (ASCP)
>
>IHC Lab Manager Veterinary Science
>
>Animal Disease Research and Diagnostic Lab
>
>South Dakota State University
>
>Box 2175 North Campus Drive
>
>Brookings SD 57007
>
> 
>
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