[Histonet] melanin pigment

[Perry, Margaret]

Mel wrote: 

I don't recall this question, but I would be interested in how you get
around the problem of epidermal pigmentation if you are looking for
protein expression in the epidermis; do you bleach the tissue (and if
so, does that affect antigenicity of the proteins), or can you choose a
detection chromogen that has a wild color, like a Barney purple color? 

Thanks for your help.

Mel

 

We use Vectors Nova Red and it works well with melanin and also
hemosiderin.

 

Margaret Perry HT (ASCP)

IHC Lab Manager Veterinary Science

Animal Disease Research and Diagnostic Lab

South Dakota State University

Box 2175 North Campus Drive

Brookings SD 57007