[Histonet] Gomori's trichrome problem

Wed Oct 25 14:21:15 CDT 2006

Distilled water is a poor rinse for trichromes; 0.5% aqueous acetic acid is
MUCH better.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Kathy.Johnston <@t> CLS.ab.ca
Sent: Wednesday, October 25, 2006 1:21 PM
To: Allison_Scott <@t> hchd.tmc.edu; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Gomori's trichrome problem

Hi Allison!

Without knowing which dyes your kit is employing, I would have to say the
lack of an aqueous rinse of some sort is your culprit.  Regardless whether
you have an Aniline Blue or Light green collagen stain component, all of the
dyes in your typical Gomori one step are far more soluble in water than
alcohol.  The Aniline Blue is probably the worst of the batch being barely
soluble in alcohol.  We use a 1% Acetic Acid rinse (approx 5 dips) to rinse
most of the dyes off before putting it in 95% ETOH then 100% ETOH and up to
Xylene. We also have charged slides in our runs and they work fine for us.

If you don't want to stock that extra reagent, just give them a quick swish
in dH2O before you run them up thru ETOH.

You will also see this phenomenon in your Retics if you forget to rinse off
your Nuclear fast red (found that out the hard way!).

Hope this might help!  Good Luck!

Kathy Johnston
Tech II Special Stains
Anatomic Pathology
Calgary Laboratory Services
#9 - 3535 Research Road NW
Calgary, AB.
403-770-3572

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Scott,
Allison D
Sent: October 25, 2006 10:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gomori's trichrome problem

Hello to all in histoland.  We are currently having a problem that has just
cropped up within the last few weeks with our gomori's one step trichrome
stain.  It is being done as the procedure says, but when the slides come out
of the one step solution and they are rinsed in 100% alcohol, they have a
white filmy background.  I think that it has something to do with the
sections being on a charged slide.  The only thing that we are not doing
according to procedure is not going directly to 0.5% acetic acid solution
after the one step solution.   Any help will be appreciated.  JACHO is in
the house, I hope that they don't come in my area.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas

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