[Histonet] Gomori's trichrome problem

[Mitchell Jean A.]

Just curious - are you purchasing your one-step trichrome solution or
making it yourself?  I have had problems in the recent past w/purchased
one-step trichrome.  And not just from one vendor but several that shall
for the time being remain un-named suppliers.

Jean Mitchell
UWHC
Neuromuscular Laboratory
Madison, WI

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Wednesday, October 25, 2006 11:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gomori's trichrome problem


Hello to all in histoland.  We are currently having a problem that has
just cropped up within the last few weeks with our gomori's one step
trichrome stain.  It is being done as the procedure says, but when the
slides come out of the one step solution and they are rinsed in 100%
alcohol, they have a white filmy background.  I think that it has
something to do with the sections being on a charged slide.  The only
thing that we are not doing according to procedure is not going directly
to 0.5% acetic acid solution after the one step solution.   Any help
will be appreciated.  JACHO is in the house, I hope that they don't come
in my area.
 

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas

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