[Histonet] Gomori's trichrome problem

Scott, Allison D Allison_Scott <@t> hchd.tmc.edu
Wed Oct 25 11:50:41 CDT 2006


Hello to all in histoland.  We are currently having a problem that has just cropped up within the last few weeks with our gomori's one step trichrome stain.  It is being done as the procedure says, but when the slides come out of the one step solution and they are rinsed in 100% alcohol, they have a white filmy background.  I think that it has something to do with the sections being on a charged slide.  The only thing that we are not doing according to procedure is not going directly to 0.5% acetic acid solution after the one step solution.   Any help will be appreciated.  JACHO is in the house, I hope that they don't come in my area.
 

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas

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