[Histonet] double staining

[Liz Chlipala]

If I remember correctly from Chris van der Loos lecture and his book on
multiple staining methods if the two antigens are co-localized then DAB is
not a suitable chromagen. If the antigens are not co-localized then DAB
would be fine.  Please feel free to correct me if I'm remembering this
wrong. I commonly use DAB with Fast Red for antigens that will stain
different cells or different cellular components.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
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phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tyler
Sent: Wednesday, October 25, 2006 2:06 AM
To: histonet
Subject: [Histonet] double staining

Morning to All Histonetters
Request from one of the Med Techs I work with. Please can someone
forward or suggest a method for double staining on tissue sections using
DAB and AEC substrate.
Thanks
Marilyn


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