[Histonet] Safranin-O

[Geoff McAuliffe]

If such research existed Dr. Churukian would know about it. Alcian Blue 
has been the subjected to more research for this application than 
Safranin O.

Geoff

George Adams wrote:

>HISTONETTERS:
>        I am trying to find out a detailed comparison of Safranin-O
>versus
>Alcian blue stains for glycosaminoglycans and proteoglycans. 
>Specifically, does Safranin-O stain sulfated (chondroitin sulfate) or
>only non-sulfated GAGs (hyaluronic acid)?  Is there an advantage or
>disadvantage to using Alcian blue for the same glycosaminoglycans?  The
>tissue that
>one of the students in our lab will be using is from human umbilical
>cord.
>    I received a very nice and quick response from Mr. Charles
>Churukian via Mr. Matthew Frank at the 
>the University of Rochester Medical Center which is in bold below.
>
>Mr. Adams,
>
>I was just informed by Charles Churukian that Alcian Blue is the
>correct
>stain to use for an umbilical cord section.  Safranin O has been used
>for bone tissue but for this application Alcain Blue is your stain.  I
>have no info on comparison studies between the two.  
>
>Matthew Frank
>
>
>Looking forward to everyone's responses.
>
>                                                                       
>                                    George Adams, Ph.D.
>                                                                       
>                                    Research Associate
>                                                                       
>                                    University of Texas Southwestern
>Medical Center
>                                                                       
>                                    Department of Otolaryngology
>                                                                       
>                                    5323 Harry Hines Blvd.
>                                                                       
>                                    Dallas TX 75230-5433
>                                                                       
>                                    214-648-0466
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>  
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
**********************************************