[Histonet] Safranin-O

[George Adams]

HISTONETTERS:
        I am trying to find out a detailed comparison of Safranin-O
versus
Alcian blue stains for glycosaminoglycans and proteoglycans. 
Specifically, does Safranin-O stain sulfated (chondroitin sulfate) or
only non-sulfated GAGs (hyaluronic acid)?  Is there an advantage or
disadvantage to using Alcian blue for the same glycosaminoglycans?  The
tissue that
one of the students in our lab will be using is from human umbilical
cord.
    I received a very nice and quick response from Mr. Charles
Churukian via Mr. Matthew Frank at the 
the University of Rochester Medical Center which is in bold below.

Mr. Adams,

I was just informed by Charles Churukian that Alcian Blue is the
correct
stain to use for an umbilical cord section.  Safranin O has been used
for bone tissue but for this application Alcain Blue is your stain.  I
have no info on comparison studies between the two.  

Matthew Frank


Looking forward to everyone's responses.

                                                                       
                                    George Adams, Ph.D.
                                                                       
                                    Research Associate
                                                                       
                                    University of Texas Southwestern
Medical Center
                                                                       
                                    Department of Otolaryngology
                                                                       
                                    5323 Harry Hines Blvd.
                                                                       
                                    Dallas TX 75230-5433
                                                                       
                                    214-648-0466