[Histonet] EDTA decalcification solution. .

[Henry, Charlene]

You can make a 10% EDTA solution and adjust the pH to 7.4 but if the
mouse femurs have been fixed in 10% formalin, I would suggest that you
use 10% formalin saturated with EDTA. I have used both and the
morphology of the bones decalcified with EDTA/Formalin is much better
than the 10% solution of EDTA. 


Charlene Henry HT (ASCP), QIHC
Histology/Immunohistochemistry Section Head
Department of Pathology
St. Jude Children's Research Hospital
901-495-3191
fax 901-495-3100

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Fawn
Jones
Sent: Wednesday, October 18, 2006 11:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] EDTA decalcification solution. .

Hi everybody,

I am getting ready to decalcify some mouse femurs for immuno staining.  
I know I have to use an EDTA decalcifier, but I do not know how to make
it.  Does anybody have a protocol on this that they would be willing to
share?

Thanks in advance
Fawn

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